Reporter

Part:BBa_K1750001:Design

Designed by: Janelle San Juan   Group: iGEM15_ANU-Canberra   (2015-09-18)

YFP fused to CIB1 N-terminal domain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 718
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 718
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 718
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 718
    Illegal AgeI site found at 262
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Initially designed CRY2-CFP-CIB1-YFP, CRY2-YFP-CIB1-YFP, CIB1-CFP and CIB1-YFP constructs with ribosomal binding sites at the start of each fusion protein (if contained both CRY2 and CIB1 fusion proteins), HIS/MYC tags and a flexible linker between the fused proteins, and a Terminator at the end. We redesigned the construct in the order RBS-CIB1-Linker-YFP-H6 to allow purification and fluorescence measurements of individual reporter proteins. The N-terminal HIS tag also facilitated purification of the protein.


Source

CIB1 - genomic sequence of A. thaliana, YFP - genomic sequence of A. thaliana A. victoria

References